Laboratory Techniques

Human error in laboratory techniques and use of laboratory equipment is a major cause of work-related injuries and infections. Laboratory staff should adhere to approved working principles, operating equipment and biosafety guidelines.
Working with patient samples in the laboratory
Make sure that you receive samples, store samples and perform tests until the waste needs to comply with regulations, use personal protective clothing such as gloves, gowns, etc., require level I of biosecurity .
The specimen is contained in a solid, suitable hardness bottle with a screw cap

. When using a sample container, each sample pack must be packed separately.
There is a separate area for receiving samples. For contagious samples when receiving, to check and sterilize outside the package
Avoid collapsing during transportation, receipt and opening of vials to ensure biosecurity, training to follow the proper procedure for each sample
Note when using pipettes
Do not suck pipette by mouth, use support devices such as squeeze balls, ..



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All pipettes should have an impermeable cotton plug to reduce contamination of supporting devices, so use filter tips to avoid splashing the solution onto the micropipette.
The micropipette is used to aspirate solutions in the specified volume range indicated on each pipette, without any adjustment to be greater or smaller than the manufacturer's recommended limit.
After use of the pipette, it must be soaked in disinfectant solution suitable for the prescribed time.
Micropipet is always on the shelf, must calibrate periodically
Note when using biological safety cabinets
Biological safety cabinets are used when handling infectious materials, which may contaminate via the respiratory tract or create aerosols such as centrifuging, crushing, making, shaking, etc or opening boxes Contains infectious materials that have internal pressure other than the outside.
Note when using centrifuges
Depending on the type of centrifuge that the operation of the machine varies
Sample tubes that need centrifuge must be leveled before being put into the centrifuge
Place equal tubes in a symmetrical position relative to the centrifugal shaft
Sample tubes should not be made of glass or fragile material, should be made of plastic to avoid broken tubes when centrifuged at high speed.
The sample should not be filled with more than 3/4 of the centrifuge tube.
Select the appropriate rotor type, put the rotor into the centrifugal chamber and tighten.



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Start the centrifuge and proceed with the centrifuge.
At the end of the centrifugation process, it is advisable to open the tube containing the centrifugal sample in the biosafety cabinet to avoid infecting the laboratory personnel with aerosol generated.
Should clean the rotor, check, maintain the machine periodically.
Note when using the refrigerator, freezer
Need a chart to monitor the daily temperature of the refrigerator, must have an inventory of items in the freezer, refrigerator
Do not store food or drink in the laboratory refrigerator.
Depending on the cabinet temperature, select the appropriate sample canister.
Preservation of samples according to the prescribed diagram and position so that the preservation, monitoring and use are most effective.
Should defrost and clean the cabinet periodically, need to disinfect the inside of the cabinet before cleaning, use protective equipment face, durable rubber gloves.
The freezer should be placed in a cool 25OC room.
Biosafety regulations should be strictly adhered to to protect employees and the public.



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KTV. HUYNH HUONG

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