Some Situations Of Handling Biosafety Incidents In Laboratories

Incident of needle sticking in hand when handling pathogen
Tell nearby colleagues (if any).
Expose the wound (take off or tear off gloves)
Immediately put the wound under clean running water.
Allow the wound to bleed itself for a short period of time, without squeezing it.
Wash the area thoroughly with soap and clean water.
Use gauze with an appropriate disinfectant to cover the wound

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Record and report the incident to the laboratory dean.
Depending on each specific case, there is a suitable way to deal with it.
Handling incidents spilled, containing solution containing pathogens on the laboratory floor
Change gloves
Take the specimen handling tool kit
Use the tongs to hold the sample container into a yellow bag containing infectious waste or a sharp object (if any) and place it in the sharps container.
Spread absorbent paper over the area of solution that spills from outside to inside.



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Pour the decontaminant onto the poured area from outside to inside.
Allow about 30 minutes for the disinfectant to exert its bactericidal effect.
Wear new gloves.
Use absorbent paper tongs to put in infectious waste bags. Wipe the spill area with absorbent paper
Absorbent paper and sharp objects (if any) are handled strictly according to the instructions for treating infectious waste
Remove gloves. Hand washing
Record and report the incident to the laboratory dean.
Incident tube collapse containing pathogens in centrifuges
If the centrifuge is running, shut down automatically, keep the lid closed, allow 30 minutes for aerosol to settle.
Change gloves.



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Use tweezers to pick up tube debris and place it in an infectious waste container.
Wipe the chamber and rotor inside the centrifuge with absorbent paper moistened with appropriate disinfectant.
Absorbent paper and sharp objects are disposed of according to the instructions for disposing of infectious waste.
Change gloves Record the incident report to the dean.
Initial management in case of fainting
Lay casualty down, raise legs up (above head level)
Loosen victim's items or clothing on the neck, chest and abdomen.
Ensure good air circulation in the room.
After the patient has regained consciousness, follow the steps below.
Help patients sit up from prison. Can give patients a little water.



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If the patient has difficulty breathing: put the patient in a prone position on the side.
Case of exposure to HIV source
Depending on the handling situation, basically there are the following steps:
Remove foreign objects such as needles, blood tubes, lamles, razors, etc from your body. Immediately notify colleagues working nearby (if any).
Reveal the entire wound (for example, removing or tearing on gloves).
Immediately put the wound under clean running water.
To allow the wound to bleed itself in a short period of time, absolutely do not squeeze the wound to avoid making the wound wider, deeper tissue damage facilitates HIV entry more quickly.
Wash the area thoroughly with soap and clean water.
Use gauze pads with appropriate disinfectant to cover the wound.
Record and report the incident to the laboratory dean.




Check the HIV status of the source and the person exposed.
If the result is positive, follow HIV treatment.
If the exposure is negative, the source of exposure is negative (subjects are not at risk group), then follow up for 3 months after testing again for the exposed person, if negative continues for the next 3 months (6 months) , if negative, record it.
If the exposure is negative, the source of exposure is positive or negative, but the subjects are at high risk and hurt the exposed person, instruct the exposed person to Tropical Hospital to take ARV within 72 hours of exposure.
KTV. KHONG VY.

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