Direct Stool Test Technique

The direct stool test technique uses physiological saline-soluble fertilizers used to detect the mobility of unicellular activity, worm eggs, tapeworms, worm larvae and abnormal objects in the faeces.
TOOL
Microscope
Lam glass, glass leaves
Wax pencil
Wood stick
Cloth towels
Ca antiseptic solution
Clip
CHEMISTRY
NaCl 0.85%
Lugol 1%
Antiseptic solution
HEPRAINING PROCESS
Get a clean, dry glass slide. Use pencil lead to divide the slide into 3 parts. Enter patient's name in the first small box of the slide

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Apply a glass slide to the middle of 1 drop of NaCl 0.85%, at the bottom of 1 drop Lugol.
Using a wooden stick to take a little stool, dissolve the fertilizer in 0 85% NaCl drops
Continue to take stool a second time and dissolve it in Lugol drops.
Put the wooden stick in the antiseptic solution.



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Cover the glass leaf with 2 drops of stool just made
Take a sample survey on the microscope.
STANDARDS OF A GOOD COPY
Not too thick: too much stool will make the template cloudy, masking parasites, difficult to detect.
Not too thin: too few stool will not find parasites unless they are too much.
The template is moderately thick when it is visible in the newspaper under the template.
The template does not cause air bubbles, the liquid does not spill around the glass leaves
SURVEY SURVEY BELOW VIRUS VIRES
Surveying stool samples with a 10x objective, when looking for details of the shape of eggs or larvae, switch to the 40x objective
Investigate the sample in zigzag form so that no field is missed.
Note:
Should keep light just enough.
The stool sample is tested as soon as possible, so the parasite will die or change its shape, difficult to identify.
Look for worm eggs, tapeworm samples for no more than 10 hours.



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Search for a single cell for no more than 2 hours.
Mistakes need to be avoided
Spread stool thick, thin place.
If the stool is too thin or too thick, discard it and try again.
Cover the glass leaf as a template to create air bubbles
The stool solution spilled out of the glass leaf.
Forget not placing glass leaves on the stool, the stool will quickly dry, the objective will be dirty and the dye will fade quickly.
Use ordinary water to dissolve stool instead of 0.85% NaCl solution, because water will often deform or destroy the activity of the single cell.
Use more light, so keep the glass close to the glass table. Reduce glare by closing down light barriers.



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HOW TO RESPONDING THE DISCUSSION RESULTS
On the answer sheet of the stool test result, the following information must be written:
Characteristics of stool: hard, soft, viscous, ..
Color of stool: yellow, blue, black, ..
Abnormal factors visible to the eye: blood, tapeworm burning,.
Techniques used: direct soi, Kato technique, ..
Result:
Negative: no eggs and larvae found in intestinal parasite.
Positive, write down the following:
Vietnamese name and scientific name of the parasite.




Eggs, active form, stage larvae.
Infection density on the template:
Few: (+) 1-2 eggs / template
Medium: (++) 3-5 eggs / template
Multiple: (+++) over 6 eggs / template
Example: Found a fertilized roundworm egg (Ascaris lumbricoides): (+).
HOW TO HANDLING DISEASES AND USED TOOLS
Product: After finishing testing, put stool and test stick into disinfecting solution and then autoclave before discarding. If there is no steamer, boil for 30 minutes.
Tools: slide glass and foil into disinfectant solution, autoclave sterilize and wash then dry.
KTV. HUYNH HUONG.

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