Where Is The Best Skin Test?

Parasitical Worms.com Patients suspected to be fungal should be tested directly under a microscope or cultured in appropriate media to determine the biochemical properties from which the fungal species are identified. .
TESTING FOR DIAGNOSTIC TREATMENT
1. Direct test method

.
1.1. Request
Testing for pathogenic fungi is similar to testing for pathogenic bacteria Patients suspected of being fungal should be examined directly under a microscope or cultured in appropriate environments to determine the biochemical properties from which the pathogen species are identified.
In the task of fungal testing, it is necessary to pay attention to the sanitary conditions of the instrument and the surrounding air at the test site, because in the air spores and hyphae are often present.



. If you do not pay attention to clean hygiene, it is easy to infect and affect the test results.
Several factors are involved in the test results.
+ Specimens: Before testing, patients must stop antifungal drugs for at least 1-2 weeks.
+ If the medicine is not stopped, the mushroom temporarily "disappears" "diving" so the technician cannot find it.
+ The ointment exists on the skin, making it difficult to see in the field of the grease, obstructing the view of the observer
+ pigments that make the skin dyed quickly, blue or purple, red, obscure the structure of the fungus
+ Observers should have orientations on the basis of the clinical summary.
1.2. Ringworm test under a microscope.



.
1.2.1. Specimens for direct testing of ringworm can be hair, scalp, scalp, facial, leg, hand, abdomen, groin, interstitial, nail, etc suspected fungal infections and specimens are usually taken at a mushroom laboratory.
1.2.2. Instrument means.
Pointed knives, extraction knives, scissors, needles, rods, clean slides, lamees, antlers, 70o C tincture, alcohol burners.



. Microscope.
12.3. Solution, chemicals.
+ Gram staining solution for direct testing of system staining.
+ KOH 20% solution for testing fungal skin infections.
+ To make the test specimen clear, keep the specimen long for testing and training, the KOH solution has more glycerin according to the following formula or DMSO solution.
20% KOH solution:
- KOH 20 g; glycerin 20 ml + distilled water just enough 100 ml.
- DMSO solution.




Distilled water 60 ml. KOH 20 grams. Dimetyslfoxide 40 ml.
1.2.4. Method of implementation.
+ Collect specimens: use magnifying glass with magnification of 5-6 times to observe the infected area on the damaged skin and then use 70o antiseptic cotton wool to eliminate dust and dirt After using a sterile heated knife on an alcohol lamp, scrape off the skin or horns into a clean slide, use a scalpel to remove the alcohol, take a swab, if it's hair, hair, then use scissors to cut, then cut hair or short hairs of about 0.1-1 cm put the specimen in the middle of the slide.



.
+ When the patient is done taking the sample 1-2 drops of 20% KOH solution into the middle of the specimen. After placing on the specimen 1 sterile clean lame, use the tip of the knife to gently press down the lame to spread the specimen on the slide, leave room temperature for 45 minutes and then scan or slide the specimen slide on the alcohol lamp, we ho Go back gently for hot, not bubbling then let cool after screening.
Attention: when examining and observing the specimens, they must be uniform throughout the field to look for the mycelium. Fungal hyphae, segments of fungal hyphae or spores can show on the keratinocytes of the skin organization or stand separately from skin cells, need to distinguish mycelium from other fibers. Mycelium is often bent, soft and zigzag sometimes branched and refractive, into the thick hyphae that look like 'noodles'.
+ Reply to results:
- Soi with hyphae, hyphae or hyphae
- Or: no hyphae, no hyphae or no spores found
In case it is necessary to identify a fungus, it is required to cultivate and then identify the fungus.
1.3. Direct testing of 'system' fungi.



.
Systemic fungi are usually yeasts or filamentous fungi that cause disease in the bodies of the human body, are taken samples and then stained with gram.
1.3.1 Collect specimens:
Means for direct testing of systemic fungi.
+ The microscope has an oil objective.
+ Gram staining solution includes the following 4 solutions:
- Solution 1: crystalviolet
. Crystalvioet 2%.
. Ethyl alcohol 20%.



.
. Oxalátamonium 08%.
- Solution 2: Lugol.
. Iodine 1.3%.
. KI 2%.
.



Polyvinylpyrrolydon 10%.
- Solution 3: Alcol.
. Alcohol ethylic 95%…. 50%.
. Aceton …………………… .50%
- Solution 4: Safranine
. Safranine ……….



. 0, 25%.
. Alcohol 95 ……… 10%.
Cypress oil for soi and xy len to wipe the template.
Usually sent by medical departments directly from the patient to the mushroom laboratory in sterile cotton swabs, test tubes and equipment. Samples may be sputum, tongue mucosa, throat fluids, bronchial fluid, pus, eye drops, cerebrospinal fluid, stool, vaginal fluids, urethra, blood or other infectious areas in organizations in the body or on the skin.
1.3.2.



. Making template:
Use sterile cotton swabs or sticks on clean sterile glass slide on alcohol lamp (allow to dry at room temperature).
Note: spread evenly in a thin layer on the slide with a tight line.

. Dịch vụ: Thiết kế website, quảng cáo google, đăng ký website bộ công thương uy tín