Direct Quantitative Bilirubin Assay

Define
Bilirubin is formed in the reticuloendothelial system during degeneration of old red blood cells. The heme component from hemoglobin and from other heme-containing proteins is removed, converted into bilirubin, and transported as a complex with serum albumin to the liver. In the liver, bilirubin combines with glucuronic acid to dissolve and is then transported through the bile duct and excreted in the digestive tract. Diseases or conditions through hemolysis, produce bilirubin faster than metabolism in the liver, causing an increase in unconjugated (indirect) levels of bilirubin in the circulatory system. Similarly in the case of immature liver and some other diseases where impaired bilirubin conjugation mechanism will also cause unconjugated hyperbilirubinemia

. Bile duct obstruction or damage to hepatocellular structure increases both the conjugated (direct) and unconjugated (indirect) concentrations of bilirubin in the circulatory system.
Principles
The diazo2 method of conjugated bilirubin and δ ‑ bilirubin (direct bilirubin) reacts directly with 3,5 ‑ Dichlorophenyl diazonium salt in acid buffer to form red azobilirubin.
bilirubin + 3,5-DPD azobilirubin
The color intensity of the red azo dye forms is directly proportional to the concentration of direct bilirubin (conjugated) and is measured photometrically
Comment: Under the influence of blue light, for example during newborn projection, unconjugated bilirubin is partially converted into a water-soluble isomer called photobilirubin, a substrate for bilirubin tests direct. This section was discovered by BILD2 and may result in above normal levels in healthy children
Content implementation
Serum is centrifuged at a speed of 4,000 rpm for 5 minutes.



.
Put the serum tube on the shelf.
Place the price in the position specified in the biochemical.
Conduct a sample analysis process according to the Biosytem A25 automatic biochemical testing standard.
When the analyzer is finished, the results will automatically transfer to the clinic management system to make the results publicly available for the entire laboratory and the doctor to read.
Check the result
Print and return results
Interpret and report the results
BIL - D blood
Direct bilirubin ≤ 34 µmol / L
Warning:
Factors affecting
Hemolysis: hemoglobin concentration> 15.5 µmol / l or 25 mg / dl.
Lipid index:> 750. There is a correlation of turbidity and triglycerides concentration
KTV.



. Ngoc Hanh.

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