How To Preserve Chemicals, Samples And Send Samples
In order to ensure the quality of chemicals, swabs and especially samples of helminths, such as blood, stool, and urine samples, until the testing techniques produce accurate results, they We need to have proper storage methods that do not affect the quality of the test.
How to preserve enzymes
Plasma samples used to measure enzyme activity generally can be stored at 4ᵒC for 5 days, the enzyme activity will not drop more than 10%. Particularly for LDH, do not store in the refrigerator because its activity decreases, its isoenzymes LDH4 and LDH5 are not stable in cold conditions. Acid phosphatate is stable only when plasma samples are acidified.
How to preserve substrates
The metabolites in serum or plasma are usually stable at 4ᵒC for 6 days, the concentration does not change significantly. Particularly for Triglycerides may be reduced due to hydrolyzed lipase. However, this concentration will not change if the method is analyzed through total glycerol.
Storage at room temperature may reduce the concentration of phosphate, uric acid and creatinine if the determination is based on a Jaffé reaction
How to protect proteins, antigens and antibodies
Proteins, antigens and antibodies can be stored at 4ᵒC for one week.
How to preserve hormones and cancer markers
Steroid hormones, relatively durable cancer markers, can be stored at room temperature (25ᵒC) for up to 3 days. Peptide hormones that need to be stored for more than a day must be stored in a deep refrigerator. Particularly unstable hormones are ACTH, renin, insulin, GH and calcitonin.
How to preserve samples for a long time
If specimens are to be stored for longer than the above period, the specimens should be frozen at a temperature below -20ᵒC. When needed, samples should be thawed slowly at 4-8ᵒC overnight or in a shaking bath
Note: for whole blood samples, before performing storage at negative temperature, it is necessary to perform serum and plasma separation
How to preserve urine for urine sediment testing
Urine residues must be evaluated for results in about 2-3 hours. Do not store urine samples in a refrigerator or freezing as refrigerated conditions can cause salt precipitation.
How to preserve stool samples
Sample type Purpose of use Storage conditions Storage time Storage equipment Notes
Manure Parasite / bacterial detection 18-30ᵒC <24 hours Sampling bottle Detects parasites, samples mixed with formaline 10% or PVA in a 3: 1 ratio Used for other diagnostic tests 4-8ᵒC> = 24 hours Vials
-20ᵒC or -70ᵒC <24 hours Sampling bottle For molecular biology testing only Rectal stool swabs Samples for all diagnostic tests -20 hoặcC or -70ᵒC> = 24 hours Rectal swabs in specialized transport media Only for molecular biology testing
How to preserve blood to measure blood gas and how to balance acid - base
The determination of blood gases and the acid-base balance of arterial or capillary blood should be avoided in air and should be performed immediately. If this is not done immediately, the blood sample may be placed in ice for a maximum of 2 hours.
How to send samples, stool samples
When sending samples from one place to another in the country or internationally, the specimen should be stored in a dry stone thermos. In general, the change in enzyme activity and metabolite concentration does not exceed ± 10% in about 2 days, if the sample storage temperature is below 25ᵒC.
Note: The samples sent must follow the packaging principle to ensure biosecurity.
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