Quantitative Assay Of Cholesterol In Blood
What is the definition of cholesterol?
Cholesterol is a steroid containing a tier 2 hydroxyl group at the C3 position. It is synthesized in different tissue types, but usually concentrates in the liver and intestinal wall. About 80% endogenous and 20% are derived from food.
Linear range: the range of values for which the measurement is accurate.
Limits of measurement: minimum concentration that a measurement can measure
The cholesterol ester enzyme digestion method is cleaved by the cholesterol esterase enzyme, which produces cholesterol and free fatty acids. Cholesterol oxidase catalyzes the oxidation of cholesterol to cholest-4-en-3-one and hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide acts on the oxidative coupling between 4 - aminophenazone and phenol to produce a red quinone-imine
Cholesterol esters + H20 CE cholesterol + RCOOH
Cholesterol + O2 CHOD cholest - 4 - en - 3 - one + H2O2
2H2O2 + 4- AAP + phenol POD quinone - imine dye + 4H20
The color intensity of the dye is directly proportional to the cholesterol concentration. Color intensity is determined by measuring the increase in absorbance.
Biochemical testing machine Biosytem A25. Centrifuge speed of 4000 rpm
Chemical testing firm
0.9% NaCl diluent solution
Serum, plasma, whole blood at high risk of infection, carrying out operations on patient samples in accordance with the procedures
Wear full protective clothing such as gown, gloves, masks ..
When chemicals, specimens are stuck on gloves, they must be changed immediately; If in eyes, rinse immediately under emergency eyewash
Samples after testing must be treated as infectious waste
Interpret and report the results.
CHOL blood: <200mg / dL Warning: Factors affecting Jaundice: conjugated bilirubin concentration> 274 µmol / l or 16 mg / dl.
Unconjugated bilirubin concentration> 239 µmol / l or 14 mg / dl.
Hemolysis: hemoglobin concentration> 435 olmol / l or 700 mg / dl.
Lipemia:> 2000 mg / dl. There is a correlation between turbidity and triglycerides concentration.
KTV. Ngoc Hanh . Dịch vụ: Thiết kế website, quảng cáo google, đăng ký website bộ công thương uy tín
- Parasitical Worms.com Tests to find the cause of urticaria, diagnosis of urticaria results will be available throughout the day. After the results the doctor will explain, point out the abnormal signs for your child to understand and he will prescribe medication for home. Question Hello doctor: I ...Parasitical Worms.com Adult flukes are very small, 3 - 6 mm long, with 4 suction heads and a double hook, very short neck; coal consists of 3 segments, the final flukes have several hundred eggs, size 45 x 35 mcm, very similar to Toenia spp eggs. The disease is caused by the larva Echinococcus ...Parasitical Worms.com Some diseases caused by larvae of the anisakinae family parasitize marine mammals. In humans, the parasite falls into a dead-end, or severe or severe illness depending on the place of parasite, number of larvae and tissue responses. Diagnosis is often difficult and the most ...Parasitical Worms.com Illness caused by the nematode of Angiostrongylus cantonensis parasitizes and causes disease in the meninges, invasion of the brain can lead to death. Commonly called Meningitis - brain caused by Angiostrongylus cantonensis. The causative agent of nematode ...Fascioliasis is two types of fascioliasis and small liver fluke. People are infected with food, skin. Flukes can cause hepatitis, liver tumors, liver necrosis, but fortunately, liver fluke can be cured if detected early, treated in a reputable facility with a good doctor, using drugs. Good, ...Parasitical Worms.com Diagnosis is determined by seeing sparganum larvae from the wound. Clinical and prehistoric images of frog meat, eye-copying as well as the habit of eating undercooked snakes, mice, and eels are important factors for diagnosis. Doctor: Le Thi Huong Giang Medical Consultation: ...MUSHROOM DISEASE (Aspergillus) 1. Epidemiology. Aspergillus fungus is one of the largest fungal strains, present in all over the world, there are about 100 species, currently there are about 20-30 species that cause disease in humans, important strains are A. fumigatus, A. flavus , A. niger such as ...MUSHROOM DISEASE Cryptococcosis (Tolurosis, European Blastomycois) 1. Etiology and epidemiology Cryptococcosis is also known as the European Blastomycose mycosis caused by Cryptoccocus neoformans, a thick cystic yeast, has serotypes A, D (C. neoformans var. Neoformans) and B, C ( C.neoformans var. ...MUSHROOM DISEASE Sporotrichosis (Gardener Disease) 1. Epidemiology and etiology Sporotrichosis is a chronic disease caused by Sporothrix schenckii that causes damage to the skin or internal organs (also known as gardener disease - gardener's disease). This is a dimorphic mushroom. In nature, ...CANDIDA MUSHROOM 1. Germs Candidiasis is an acute, subacute or chronic disease caused by Candida-like yeasts, mostly Candida albicans. Candidiasis is available in the body (bronchus, oral cavity, intestine, vagina, skin around the anus) normally in non-pathogenic form. When having favorable ...