Skin Tests For Fungal Diseases

Tinea is caused by a type of dermatophytosis. In addition, some yeast strains such as candida, tritrophyton, epidermophyton, microsporum, .. are also causes of disease.
Techniques to take samples and do a Pap test
Tinea usually causes disease on keratin surfaces such as skin, hair, hair, and nails

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Clinically, depending on the type of fungal infection, the image of skin lesions will have different manifestations. In order to treat good results, patients need to be detected early and treated promptly.
Testing for pathogenic fungi is similar to testing for pathogenic bacteria Patients suspected to be caused by fungi will be examined directly under a microscope or cultured in appropriate media to determine the biochemical properties from which the fungus can be identified sick.
In the task of fungal testing, attention should be paid to the hygienic conditions of the instruments and the surrounding air where the test is performed, because in the air spores and hyphae will often appear.



. If you do not pay attention to cleanliness, it is easy to lead to an infection that affects the test results.
Tools, Chemicals
Optical microscope.
Microscope slide.
Lamen.
Alcohol lamp and 90 degree alcohol
Sterilized scalpel
Nail clippers.
Tweezers hair pulling.
Select the affected area and shave the skin there. If there are multiple lesions, each takes a few.



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Leave sterile leather sample and Petri dish
Areas where the skin has an infection is not shaved.
If the nail lesions are thick and loose on the free margin: use a scalpel, scissor and cut the suppository on the nail
If the nail is broken between the nail and the nail, use a sharp knife to scrape away the keratin from under the nail
If inflammation is around the nail: Scrape skin around the dish
Add the horny layer to a sterile glass or petri dish.
Template making techniques:
Apply 1-2 drops of chemicals onto freshwater glass.
Cover the lamen
Small KOH, heat the slide over the alcohol lamp.
Microscope under 10x and 40x objectives.
Record the results on the test order sheet.
Handling tools and samples.
Get the results
Before screening we should spread the template thinly. Use objective 10 to determine the field of view briefly, then use objective 40 to identify.



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Gray mushrooms: common in palms, hyphae with baffles, short divided, dark colored.
Tinea: usually hyphae transparent, baffled, can see spores burned
Tinea versicolor: see coarse, short mycelium like shredded vermicelli and round capsule-shaped clusters of egg cells
Filling and fungal spores in hairline (Endothrix): Internal development.
See mushroom spores around the hair (Ectothrix): Phat.
Some factors related to the test results should be noted:
Samples: before testing, patients must stop antifungal medication for at least 1-2 weeks.
If the drug does not stop, the mushroom temporarily "disappears", "diving" makes the technician find it.
Ointments that exist on the skin will make the field much more obnoxious, obstructing the view of the person looking at.
Pigments will make the skin dyed blue or purple, red, obscuring the structure of the fungus.
The technician scanning should be oriented on the basis of the clinical summary.
KTV.



Nguyen Hanh.

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