Technique For Finding Hidden Blood In Stool
To detect a number of diseases in the gastrointestinal tract causing bleeding in the intestines, including colitis, gastric ulcer, worm infection, intestinal flukes need to use testing techniques to know exactly. If it is not detected in time causing prolonged bleeding, the patient will lose blood with no known cause or danger.
Techniques for finding hemoglobin in the stool by Aminopyrine
Principle: When hemoglobin is exposed to Hydrogen Peroxide, Oxygen is released, this Oxygen will affect with Aminopyrine to color.
Patient preparation: A day before the stool blood test, the patient must not eat meat, take iron-containing medicines, brush teeth vigorously causing bleeding.
10% acetic acid
Ethylic alcohol 95o
Positive control (1% solution with water-soluble blood)
Negative control (distilled water)
Mixing aminopyrine solution: 0
Get some fertilizer into the centrifuge tube. Add 7ml of distilled water and dissolve the stool.
Centrifugal 1000 rpm with time 5 minutes
Pour the supernatant water into a test tube
Slowly put into the test tube the following:
10 drops of acetic acid solution
5ml aminopyrine solution
10 drops of hydrogen peroxide
Take care not to mix substances together
Let stand for 1 minute
Read the results within 5 minutes after adding Hydrogen Peroxide.
Read the result: a positive result will appear red between two layers of solution:
Pale red: positive reaction (+)
Magenta: strong positive reaction (++)
Dark red: very aggressive reaction (+++)
No color: negative reaction (-)
Technique for detecting blood in stool by Test
Is a fast, simple, highly sensitive and accurate technique.
Principle: Hemoglobin in stool attached to anti-hemoglobin antibody is shown on the paper strip. This complex is seen by dye.
Tools and chemicals:
Commercially purchased reagents (FOB) include:
Test bars in packaging
Vials of diluted water.
Collect patient's stool in a clean, dry bottle.
Take a stool test stick, at least 3 different places on the stool sample
Put the stool into the vial of diluted stool and mix well
Tear the cover to remove the test strip.
Add 2 drops of stool solution to the test rod into the well with the letter S.
Read results in 5-10 minutes.
Read the results:
Positive: 2 lines (1 control line, 1 line of the test sample)
Negative: 1 control line
If only 1 line of the sample shows up, the test is invalid.
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