Testing For Toxoplasma Gondii Parasite And What You Need To Know

ELISA Kit Toxoplasma gondii IgG, IgM
Toxoplasma gondii is a protozoan parasite. They live in animals like cats, dogs, mice, ... and people are also places for them to parasitize and cause disease. Because the symptoms of the disease are sometimes nonspecific (i.e., anemia, splenomegaly, jaundice, fever, hepatomegaly, lymphadenitis and vomiting), congenital Toxoplasma is easily misdiagnosed on a clinical basis, even sick children have the general form of the disease


Toxoplasmosis must also be considered in differential diagnosis in any immunosuppressive patient who has clinical or laboratory evidence of damage to the central nervous system These organisms are one of the most common human pathogens worldwide.
Who often gets Toxoplasma (infected with cat parasite)?
Toxoplasma is a common parasite in the world.


Testing For Toxoplasma Gondii Parasite And What You Need To Know

. Toxoplasma infection due to infection or from birth (congenital). Millions of people infected with Toxoplasma only show few symptoms because healthy people often have a high immune system to fight off the parasite that causes the disease.
What are the signs and symptoms of Toxoplasma infection (cat parasitic infection)?
Most healthy people usually do not show symptoms. The remainder may have flu-like symptoms, including redness, fever, fatigue, muscle aches, headache, and lymphadenopathy.
Many patients who have an early infection during pregnancy will cause miscarriage or stillbirth
The surviving children are often born with serious problems, such as: convulsions, splenomegaly, jaundice, jaundice, severe eye infection, hearing loss, mental disorders
If you have any questions or symptoms, go to top hospitals and parasite clinics for timely advice and treatment.
Preparation for testing for Toxoplasma gondii IgG, IgM
1. Micro-well tray covered with Toxoplasma gondii antigen (inactivated): 96 wells in 12 bars and 8 micro wells, preserved in aluminum bags with preservatives.
2.


Testing For Toxoplasma Gondii Parasite And What You Need To Know

. Diluent Type I Serum: Ready for use.
3. Calibrator: human fibrin serum or plasma. with a specific element printed on the bottle label Calibrators are used to calibrate tests to account for daily fluctuations due to temperature and other test conditions.
4. Positive Control: human fibrin serum or plasma. Positive Control is used to control the positive test interval.
5. Negative Control: human fibrin serum or plasma is reduced.


Testing For Toxoplasma Gondii Parasite And What You Need To Know

. Negative Control is used to control the negative test range.
6 Horseradish-peroxidase (HRP) Conjugate: Ready to use. Goat antibodies are resistant to human IgG, containing proclin (0.1%) and gentamicin for storage.
7. Chromogen / SubstrateSolutionTypeI: (TMB), ready to use. Reagents should be closed when not in use. If no evaporation occurs, a precipitate may form in the reagent wells.
8.


Testing For Toxoplasma Gondii Parasite And What You Need To Know

Wash Buffer Type I (20X concentrate): Dilute 1 part concentrated solution + 19 parts distilled or deionized water.
9. Stop Solution: Ready to use, containing 1N H2SO4 solution.
Testing technique ELISA Toxoplasma gondii IgG, IgM
1. Break the number of wells to be used in the support frame.
2. Dilute test serum, Calibrator and Control ratio 1:21 (eg 10 μl + 200 μl) in Serum Diluent. Mix
3. For each well, add an appropriate 100 μl Calibrator, Control and diluted patient serum.


Testing For Toxoplasma Gondii Parasite And What You Need To Know

. Add 100 μl of Serum Diluent to the reagent well.
4. Incubate the wells at room temperature (21-25 ° C) for 25 minutes.
5. Use a semi-automatic washing device to add 250-300 μl Wash Buffer diluted to each well. Suck all liquids Repeat the washing process four times (a total of five (5) washing) for the semi-automatic devices. After the final wash, blot the tray on paper towels to remove all liquid from the well.
6. Put 100μl Conjugate into each well.


Testing For Toxoplasma Gondii Parasite And What You Need To Know

.
7. Incubate the wells at room temperature (21-25 ° C) for 25 minutes.
8. Repeat the washing step as described in step 5
9. Add 100 µL Chromogen / Substrate Solution (TMB) to each well, including reagent wells, maintaining the same small velocity between the wells.
10. Incubate the wells at room temperature (21-25 ° C) for 10 minutes.
11. Stop the reaction by adding 100 µL Stop Solution including the reagent well.


Testing For Toxoplasma Gondii Parasite And What You Need To Know

. Shake the solution in the well and then put in the reading machine results.
Interpret the results
The patient's ISR (Immune Condition Rate) values are explained as follows:
ISR Result Explained
≤090 negative No antibody detected Toxoplasma gondii by ELISA test.
0.91-1.09 Doubt The sample should be rechecked.
≥1.10 positive Indicates the presence of Toxoplasma gondii resistance by ELISA test. Manifestations of the current or previous infection. Patients may be at risk of infection with Toxoplasma gondii, but not necessarily present infection.


Testing For Toxoplasma Gondii Parasite And What You Need To Know


How to prevent Toxoplasma gondii disease
Part of the treatment will also prevent the disease from getting worse. Toxoplasma infection (Toxoplasma parasite) can be limited if you: regularly perform blood tests to sign, turn off.

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