Testing Procedure By Elisa For Strongyloides Strongyloidiasis

Strongyloides IgG ELISA Kit is an enzyme-linked immunosorbent immunosorbent assay (ELISA) used to qualitatively detect IgG antibodies against Strongyloides stercoralis in human serum samples. This technique makes the diagnosis of human strongyloidiasis accurate and quick. This is a blood test used only by a medical technician.
PRINCIPLES FOR TESTING
The well is coated with the Igy Strongyloides antigen. After incubation of the patient's serum, this step has antibodies in the serum, it will bind to the antigen coated in the well

. Next, the wells must be washed off the rest of the sample, and added to the conjugated enzyme.
Continue incubation a second time, using small conjugated enzymes into the well, it will bind to antibodies in the well (if any). Then wash a second time Tiny Tetramethylbenzidine colorant or TMB Thanks to the presence of conjugated enzymes, the catalyst Peroxidase will use to change the color to green. Small solution stops reaction when sufficient time has elapsed.



. Finally, read the results of the test with the naked eye and with an ELISA meter with OD units.
TESTING PROCESS
Make sure that the samples and reagents are stored at room temperature (15-25 ° C).
After washing, you need to beat the well upside down with a towel.
The positive and negative testimonials have been prepared from an American chemical supplier.
Break off the number of wells needed (two for the control plus the number of samples) and place in the micro-holder bar
Dilute the patient serum at a ratio of 1:64 with diluted buffer (Example: 5 μl serum with 315 μl dilution buffer) Add 100 µl (or 2 drops) of negative control to well # 1, 100 µl of positive control into well # 2 and 100 µl of diluted test sample (1:64) into the remaining wells.
Incubate at room temperature for 10 minutes, then wash.
Wash and smash the well upside down on a tissue.
Add two drops of conjugated enzyme to each well.



.
Continue incubation for 5 minutes at room temperature.
Wash twice. Beat the well upside down on a white paper towel.
Continue adding 2 drops of colorings
Incubate at room temperature for 5 minutes.
Put 2 drops of solution to stop reaction into each micro well.
Mix the solution in the well for 15 seconds.
Small solution stopped reaction when sufficient time.
Read the test results within an hour later.
The washing step consists of 3 sprays of washing fluid to fill each well and pour out.



. When possible, avoid foaming in wells as this may affect the end result.
RESULT
See with the naked eye: Look at each well on a white background (such as a tissue) and note it as non-reactive, +, ++ or +++
Measured by ELISA machine with OD unit: read level 0 with air. Read dual wavelength at 450 / 620-650nm.
How to prevent strongyloidiasis
Each person should note the following issues to limit infection, re-infection and transmission of strongyloidiasis in the community:
Hygiene and prevention of epidemics: good disposal of manure, water and garbage in the living environment. Clean the environment near the home, in the house and play areas of young children
Personal hygiene: always wash your hands before eating, when preparing food, after using the toilet, do not eat raw vegetables without being washed
Periodic examination of helminths and once every 6 months for deworming.
Make sure to use labor protection when working in contact with the soil, especially in areas contaminated with human feces.
Strengthen the body's resistance by adding fresh, clean vegetables and exercising daily.
To raise people's awareness about community sanitation, construction of sewers and waste water treatment systems up to standards.
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