Testing Technique For Lymphatic Filariasis In The Blood

The following techniques can be used to find threadworm larvae
Direct scan to see larval motility. Specimens are blood, lymphatic fluid or urine sediment.
Making thick blood drops, dye Giemsa see the shape and structure of the nucleus. Microscopic examination.
Knott technique is the technique of concentrating larvae.
Filter through a membrane with a Nuclepore filter, carried out when infected with worms with only low embryo density in the blood.
Quick test for the diagnosis of filariasis: simple and fast operation, with high sensitivity.
Lymph node biopsy for adult lymphatic filariasis


Serological test
Knott technique
The Knott technique is a technique of concentrating only worms in the blood, used in mild infections, with few parasites. This technique uses a large amount of blood, red blood cells are dissolved by the formol solution, then centrifuged.


Testing Technique For Lymphatic Filariasis In The Blood

. Worms will concentrate only at the bottom of the test tube.
Tool
Microscope
Sterile needles
Blood tubes with heparin
Microscope slide
Test tubes or centrifuge tubes
Centrifuge
Pasteur straws
Chemistry
Forrmol 2% solution (mix carefully stored in a vial with a lid, use within 24 months).
Implementation process
In a centrifuge tube 10 ml of formol solution 2%
Add 1ml of fresh blood or blood with anticoagulant
Mix thoroughly centrifuged at 300 rpm for 5 minutes
Remove the floating water
Use a Pasteur straw to mix the residue and drip 1 drop onto the glass lame
Cover the glass leaf on the scale
Screening for worm larvae only under KHV
If threadworm larvae are found, add a thick drop specimen, dye giemsa for identification.
This method is quick, simple, but the larvae cannot be seen because it is dead.
How to prevent filariasis
You can control worms only if you apply the following:
The most effective way is to avoid mosquito bites
Air-conditioners should be placed in bed or mosquito nets to avoid mosquitoes
Do not travel to areas where filariasis is common Wear long clothes if you live near areas where there are mosquitoes or when traveling to areas where threadworm disease
Using an insecticide spray, apply mosquito repellent between dusk and dawn.
KTV. HUYNH HUONG.

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