The Process Of Testing Blood For Dog Flukes (elisa Echinococus Igg) With Chemicals Imported In The Us
Finding human tapeworms with ELISA by taking blood samples helps to accurately diagnose hidden dog fluids in the body that few people expect. Echinococcus ELISA Test is an enzyme-linked immunosorbent immunosorbent assay (ELISA) for qualitative screening for IgG antibodies to Echonococcus sp. in human serum samples
Echinococcosis (hydatidosis) is an infection caused by tapeworm in the genus Echinococcus. Humans are a potential intermediate host and can be infected by ingesting tapeworm eggs excreted from the faeces of an infected animal. This disease is called hydatidosis, or tapeworm disease.
PRINCIPLES FOR TESTING
Microwaves are coated with crude antigen from Echinococcus. In the first incubation step with patient serum, if any antibodies appear, they will bind to the antigen-covered wells during the first incubation.
MATERIALS AND COMPOSITION
Materials are provided with the test kit
Micro-well tray: contains Echinococccus antigen - 96 wells in a micro-holder
Conjugated Enzyme: A bottle containing 11ml of Protein A conjugated to peroxidase
Positive: One vial contains 1 ml of diluted positive rabbit serum.
Negative control: A vial containing 1 ml of negative human serum is diluted.
TMB substrate solution: One bottle contains 11 ml of tetramethylbenzidine (TMB) colorings.
20X Concentrated Washing Solution: A bottle containing 25ml of buffer solution and concentrated surfactant.
Dilution Buffer: Two vials containing 30 ml of buffer protein solution.
Solution Stop reaction: One bottle contains 11 ml of 0,73 M solution of phosphoric acid
- Break off the sufficient number of wells to be placed in the micro-well retention frame.
- Add 100 µl (or two drops) of negative control to well 1, 100 µl of positive control into well 2 and 100 µl of diluted test sample (1:64) in the remaining wells
Note: the yin and yang provided are diluted earlier No further dilution.
- Incubate for 10 minutes at room temperature 15-25 ºC
- Shake well and wash 3 times with diluted wash buffer.
- Add 2 drops of conjugated enzyme to each well.
- Incubate for 5 minutes at room temperature
- Shake well and wash 3 times with wash buffer.
- Eliminate washing water by beating the well on paper towels.
- Add 2 drops of colorant to each well.
- Incubate for 5 minutes at room temperature.
- Add 2 drops Solution Stop reaction and mix by inverting the micro-well holding frame
SURVEYS AT THE WASHING step can result in fake DUAL RESULTS
See with the naked eye: Look at each well on a white background (such as a tissue) and note it as non-reactive, +, ++ or +++.
ELISA reader: Zero level with air. Read dual position 450 / 620-650 nm wavelength.
Negative tones are too dark when testing.
Reason: washing is not enough
Solved: wash more thoroughly. Remove the liquid from the well by banging on the absorbent towel.
. Do not allow micro-well to dry.
Positive:> 03 OD units
Negative: <0.3OD. A negative OD result indicates that the patient does not have enough detectable antibodies. This may be due to the patient's uninfected or poor immune response. Interpret the Results - read by eye Compare the result with the control, the test result is positive when color is noticeable. Do not add acid to the samples or any reagents. PRESERVATION Store bottled ingredients, microtubes and reagents at 2 - 8ºC. The diluent buffer container may be stored at room temperature.
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