What Is The Quantitative Glucose Test?
What is the definition of glucose?
Glucose is the main source of energy for body cells and the maintenance energy for the brain and nervous system, the supply must be available, stable and maintained at a relatively constant constant level in the blood. . Glucose oxidation is the main source of energy for the body's cells. Glucose from food energy is converted into glycogen and stored in the liver or into fatty acids stored in adipose tissue. The glucose level is controlled at a narrow level by many hormones, the most important of which is the hormone in the pancreas
Principles
UV test
The enzyme reference method uses hexokinase. Hexokinase catalyzes the phosphorylation of glucose into glucose - 6 - phosphate through ATP.
Glucose + ATP HK G - 6 - P + ADP
glucose - 6 - phosphate dehydrogenase oxidizes glucose - 6 - phosphate to gluconate - 6- phosphate in the presence of NADP
G -6-P + NADP + G-6PDH gluconate-6-P + NADPH + H +.
.
Equipment
Biochemical testing machine Biosytem A25
Centrifuge speed reaches 4000 rpm.
Reagents
Chemical testing firm.
0.9% NaCl diluent solution
Content implementation
Centrifuge blood tube for 5 minutes at a speed of 4000 rpm.
Place this blood tube in a position on the rack (rack) for patient analysis
Place this price on the position to run the model in the machine system.
Analyze the sample using the Biosytem A25 biochemical manual process.
When the analyzer is finished, the results will automatically transfer to the network
Check the result
Print and return results
Interpret and report the results
Blood glucose: 74 - 106 mg / dL
Warning
When the patient's test results are <50 mg / dl or> 270 mg / dl, it is necessary to notify the treating physician directly.
Factors affecting
Jaundice: Conjugated bilirubin concentration and unconjugated bilirubin concentration> 1026 µmol / l or 60 mg / dL.
.
Hemolysis: hemoglobin concentration> 621 µmol / l or 1000 mg / dl.
Lipemia:> 1000mg / dL. There is a correlation between turbidity and triglycerides concentration.
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