All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative

CLO-test is a test that finds H. pylori in the stomach that is not known to everyone. In particular, CLO-negative test is a concept that still makes many people wonder. Here is the answer advice.
Peptic ulcer disease hp
Helicobacter pylori, commonly known as H. pylori, is a bacterium that causes infections of the stomach and small intestine. In 1982 by two Australian researchers discovered the bacteria and found that it caused peptic ulcer disease.
A stomach ulcer is an open sore in the lining of the stomach or upper small intestine (colon)

H.


All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative

pylori also increases the risk of developing stomach cancer.
Some causes of H. pylori infection:
Do not clean your hands before eating, after using the toilet
Eat untreated food that is safe and clean
Drinking water is not clean enough and safe
Some symptoms of H. pylori infection:
Hp bacteria are the main cause of gastritis
Many people with H. pylori do not have any signs or symptoms. However, if people become infected with H. pylori, they may have symptoms such as:
Dull or burning pain in the upper abdomen The pain is sometimes worse at night or on the stomach
Anorexia
Chills
Nausea or indigestion
Flatulence, bloating, heartburn
Black or bloody stool
Ulcers can lead to serious complications if left untreated, including:
Internal bleeding can be life-threatening
Perforation of the stomach can lead to infection
Scar tissue can block the stomach or intestines, preventing food from being eliminated
These complications require immediate medical attention

Methods for testing stomach HP
Pylori can be detected during blood antigen tests, breath tests, stool antigen tests or gastric biopsies and tests.


All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative


Ulcers, gastritis and stomach cancer are often diagnosed with a combination of the following methods:
History: past medical problems and symptoms or family genetic factors
Physical examination: examination and abdominal listening
X-ray inside stomach
Endoscopy: Doctors inside the stomach with a scope with cameras, lights ... to identify the condition of the wound, detect ulcers and even signs of precancer.
X-rays to detect gastric diseases
What is CLO-test?
Campylobacter-Like Organism (CLO-test) test to diagnose whether a patient is infected with Helicobacter pylori (H. pylori).
In order to obtain a CLO-test sample, a gastroscopy may be needed for biopsy.
The CLO-test has a high false negative rate and needs to be reassessed with other methods such as the current infection marker test (CIM), polymerase chain reaction test (PCR) to diagnose infection.


All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative

Helicobacter pylori (H. pylori).
All three methods have high accuracy in diagnosing H. pylori infection when stomach ulcers are present.
CLO-test procedure
Stomach biopsy
Two biopsy samples were taken from the stomach through upper gastrointestinal endoscopy. These samples are used to detect H. pylori infection by CLO-test.
CLO-test
The CLO-test is performed by placing a biopsy into a test environment containing urea and red phenol and checking the pH index.


All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative


The test environment components are as follows:
2 g Urea, 2,5 ml of red phenol water 0,4% wt / vol
0.14 g NaH 2 PO 4 .H 2 O 10 mmol
Distilled water up to 100 ml (pH 6.3-6.5)
0.4 g of jelly (Merck).
All chemicals were prepared and used within a month. Reference strains (Helicobacter pylori J99 for positive CLO test and clinical E.


All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative

coli for negative CLO test) were used to check for CLO test before use.
Check the result of CLO-test
Enzyme (yeast) is produced by urea hydrolyzed bacteria to release CO2 and NH3. The release of ammonia raises the pH of the test environment and changes the color of the pH indicator from yellow to pink or red. The change in color shows that the biopsy sample is positive for H. pylori.
CLO-test is negative and CLO-test is positive
Positive CLO-test
A positive CLO-test means that there is a pink or red change in the pH indicator when testing a biopsy. This proves the presence and activity of H. pylori bacteria in the stomach environment.


All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative

Thus, the treatment of peptic ulcer focuses on eradicating H. pylori.
Blood tests are needed to find out if there is hp infection
Negative CLO-test
In contrast, the negative CLO-test is that on the indicator bar, the yellow indicator remains the same color, with no change to red or pink. This means that there is no H. pylori infection and activity in the stomach environment. The cause of H. pylori can be excluded from peptic ulcer. The patient uses other treatments.


All Information Needs To Know About Clo-test, Clo-test Negative And Pseudo-negative


CLO-test false positive / false negative
In some cases, the CLO-test may give a false positive or false negative result.
The nature of the CLO-test gastritis test only shows that the patient's stomach contains Urease - H. Pylori bacteria secreted when it exists in the stomach. Actually, some bacteria.

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